UPLC-Q-TOF-MS Analysis of Chemical Constituents of Paederiae Herba for Folk Use / 中国实验方剂学杂志

Objective:The chemical constituents of ethanol extracts from Paederia scandens，P. scandens var.tomentosa，P. stenophylla and P. foetida used in folk were identified. And the differences of chemical constituents among the four kinds of ethanol extracts were compared. Method:The chemical constituents of four species of Paederia were qualitatively and rapidly analyzed by UPLC-Q-TOF-MS. The data were analyzed manually and matched through databases to determine the differences among the chemical constituents of four species of Paederia. Result:Twenty-three compounds were identified from four species, including 12 iridoid glycosides，such as paederoside，paederosidic acid，asperuloside，asperulosidic acid，paederosidic acid methyl ester and monotropein.5 quinic acid derivatives, such as 1-O-caffeoylquinic acid，chlorogenic acid，cryptochlorogenic acid,and 5 flavonoid glycosides，such as quercetin-3-O-rutinoside-7-O-glucoside,quercetin-3-O-rutinoside-7-O-xyloside，rutin and simple compoundkinsenoside. Conclusion:Based on the UPLC-Q-TOF-MS analysis of the chemical components of four kinds of Paederia，the four peaks with the highest response intensity were paederoside，paederosidic acid，asperuloside and asperulosidic acid，all of which were iridoid glycosides.

PCA affects expression of TF in HUVECs through NF-κB and AP-1 sig-naling pathways / 中国病理生理杂志

AIM:To explore the role of nuclear factor-κB(NF-κB)and activator protein-1(AP-1)signaling pathway in the inhibitory effects of Agkistrodon acutivirus protein C activator(PCA)on lipopolysaccharide(LPS)-induced tissue factor(TF)expression in human umbilical vein endothelial cells(HUVECs).METHODS: The viability of the HUVECs was measured by MTT assay.The protein distribution of tumor necrosis factor-associated factor 6(TRAF6)in the cells was detected by immunohistochemical staining.The protein expression of NF-κB p65,TF,c-Fos and c-Jun was deter-mined by Western blot.The mRNA expression of TF in the HUVECs was detected by qPCR.The content of TF in the me-dium of each group was measured by ELISA.RESULTS:Compared with the control group,the viability of the HUVECs in LPS group decreased significantly(P<0.01), obvious yellow dye particles appeared in the cytoplasm, cytoplasmic stai-ning deepened,and the average absorbance of TRAF6 was increased(P<0.01).The protein expression of NF-κB p65, c-Jun and c-Fos were significantly increased(P<0.01).The expression of TF at mRNA and protein levels were signifi-cantly increased(P<0.01).Compared with the LPS group,the cell viability in PCA +LPS group was slightly increased (P<0.05),the cell morphology was normal,cytoplasmic yellow dye particles were not obvious, and the average absor-bance of TRAF6 was significantly lower than that in LPS group(P<0.01).The protein expression of NF-κB, c-Jun and c-Fos was significantly decreased(P<0.01),and the expression of TF at mRNA and protein levels were decreased(P<0.01).CONCLUSION:PCA significantly reduces the damage of HUVECs induced by LPS.The mechanism may be a-chieved by reducing the activation of TRAF 6,NF-κB and AP-1 nuclear transcription factors,thereby reducing the release of tissue factor.